Kev Taw Qhia Txog Cov Peptide Trace Mineral Chelates Me Me
Tshooj 1 Keeb Kwm ntawm Cov Khoom Siv Ntxiv Rau Cov Pob Zeb
Nws muaj peev xwm muab faib ua plaub tiam neeg raws li kev txhim kho ntawm cov khoom siv ntxiv rau hauv cov zaub mov:
Tiam thawj: Cov ntsev inorganic ntawm cov zaub mov tsis zoo, xws li tooj liab sulfate, ferrous sulfate, zinc oxide, thiab lwm yam; Tiam thib ob: Cov ntsev organic acid ntawm cov zaub mov tsis zoo, xws li ferrous lactate, ferrous fumarate, tooj liab citrate, thiab lwm yam; Tiam thib peb: Amino acid chelate pub qib ntawm cov zaub mov tsis zoo, xws li zinc methionine, hlau glycine thiab zinc glycine; Tiam plaub: Cov ntsev protein thiab cov ntsev me me peptide chelating ntawm cov zaub mov tsis zoo, xws li protein tooj liab, protein hlau, protein zinc, protein manganese, peptide me me tooj liab, peptide hlau me me, peptide zinc, peptide me me manganese, thiab lwm yam.
Cov tiam neeg thawj zaug yog cov zaub mov inorganic trace, thiab cov tiam neeg thib ob txog plaub yog cov zaub mov organic trace.
Tshooj 2 Vim Li Cas Thiaj Xaiv Cov Peptide Chelates Me Me
Cov peptide me me chelates muaj cov txiaj ntsig zoo li nram no:
1. Thaum cov peptides me me chelate nrog cov hlau ions, lawv nplua nuj nyob rau hauv cov ntaub ntawv thiab nyuaj rau saturation;
2. Nws tsis sib tw nrog cov amino acid channels, muaj ntau qhov chaw nqus thiab ceev ceev nqus;
3. Siv zog tsawg dua; 4. Muaj ntau qhov chaw tso quav, siv tau ntau dua thiab ua tau zoo dua rau kev tsim tsiaj txhu;
5. Tshuaj tua kab mob thiab tshuaj antioxidant;
6. Kev tswj hwm lub cev tiv thaiv kab mob.
Muaj ntau txoj kev tshawb fawb tau qhia tias cov yam ntxwv saum toj no lossis cov teebmeem ntawm cov peptide me me chelates ua rau lawv muaj kev cia siab dav dav thiab muaj peev xwm txhim kho, yog li peb lub tuam txhab thaum kawg txiav txim siab coj cov peptide me me chelates ua lub hom phiaj ntawm lub tuam txhab kev tshawb fawb thiab kev txhim kho cov khoom siv organic trace mineral.
Ntu 3 Kev ua haujlwm ntawm cov peptide me me chelates
1. Kev sib raug zoo ntawm cov peptides, amino acids thiab cov protein
Qhov hnyav molecular ntawm cov protein yog tshaj 10000;
Qhov hnyav molecular ntawm peptide yog 150 ~ 10000;
Cov peptides me me, uas tseem hu ua cov peptides molecular me me, muaj 2 ~ 4 amino acids;
Qhov nruab nrab molecular hnyav ntawm cov amino acids yog li 150.
2. Kev sib koom ua ke ntawm cov amino acids thiab peptides chelated nrog cov hlau
(1) Cov pab pawg sib koom tes hauv cov amino acids
Cov pab pawg sib koom ua ke hauv cov amino acids:
Cov amino thiab carboxyl pawg ntawm a-carbon;
Cov pab pawg sab ntawm qee cov a-amino acids, xws li sulfhydryl pab pawg ntawm cysteine, phenolic pab pawg ntawm tyrosine thiab imidazole pab pawg ntawm histidine.
(2) Cov pab pawg sib koom tes hauv cov peptides me me
Cov peptides me me muaj ntau pawg sib koom ua ke dua li cov amino acids. Thaum lawv chelate nrog cov hlau ions, lawv yooj yim dua rau chelate, thiab tuaj yeem tsim multidentate chelation, uas ua rau chelate ruaj khov dua.
3. Kev ua haujlwm zoo ntawm cov khoom me me peptide chelate
Lub hauv paus theoretical ntawm cov peptide me me txhawb kev nqus ntawm cov zaub mov me me
Cov yam ntxwv ntawm kev nqus ntawm cov peptides me me yog lub hauv paus theoretical rau kev txhawb nqa kev nqus ntawm cov ntsiab lus kab. Raws li txoj kev xav ntawm cov protein metabolism ib txwm muaj, cov tsiaj xav tau protein yog qhov lawv xav tau rau ntau yam amino acids. Txawm li cas los xij, nyob rau xyoo tas los no, kev tshawb fawb tau qhia tias qhov piv ntawm kev siv cov amino acids hauv cov pub los ntawm ntau qhov chaw sib txawv, thiab thaum cov tsiaj tau pub nrog cov zaub mov homozygous lossis cov zaub mov tsis muaj protein amino acid sib npaug, qhov kev ua tau zoo tshaj plaws tsis tuaj yeem tau txais (Baker, 1977; Pinchasov et al., 1990) [2,3]. Yog li ntawd, qee cov kws tshawb fawb tau muab tso rau pem hauv ntej lub tswv yim tias cov tsiaj muaj peev xwm nqus tshwj xeeb rau cov protein tsis hloov pauv nws tus kheej lossis cov peptides cuam tshuam. Agar (1953) [4] thawj zaug pom tias txoj hnyuv tuaj yeem nqus thiab thauj diglycidyl tag nrho. Txij thaum ntawd los, cov kws tshawb fawb tau muab tso rau pem hauv ntej ib qho kev sib cav hais tias cov peptides me me tuaj yeem nqus tau tag nrho, lees paub tias cov glycylglycine tsis hloov pauv raug thauj thiab nqus tau; Muaj ntau cov peptides me me tuaj yeem nqus tau ncaj qha rau hauv kev ncig xyuas hauv daim ntawv ntawm peptides. Hara et al. (1984)[5] kuj tau taw qhia tias cov khoom kawg ntawm cov protein hauv txoj hnyuv zom zaub mov feem ntau yog cov peptides me me es tsis yog cov amino acids dawb (FAA). Cov peptides me me tuaj yeem hla dhau cov hlwb mucosal hauv plab hnyuv tag nrho thiab nkag mus rau hauv cov ntshav ncig (Le Guowei, 1996)[6].
Kev Tshawb Fawb Txog Kev Ua Haujlwm ntawm Cov Peptide Me Me Txhawb Kev Nqus Tau Cov Minerals, Qiao Wei, et al.
Cov peptide me me chelates raug thauj thiab nqus tau hauv daim ntawv ntawm cov peptides me me
Raws li txoj kev nqus thiab thauj thiab cov yam ntxwv ntawm cov peptides me me, cov zaub mov me me chelate nrog cov peptides me me ua cov ligands tseem ceeb yuav raug thauj mus rau tag nrho, uas yog qhov zoo dua rau kev txhim kho lub zog ntawm cov zaub mov me me. (Qiao Wei, et al)
Kev Ua Haujlwm Zoo ntawm Cov Peptide Chelates Me
1. Thaum cov peptides me me chelate nrog cov hlau ions, lawv nplua nuj nyob rau hauv cov ntaub ntawv thiab nyuaj rau saturation;
2. Nws tsis sib tw nrog cov amino acid channels, muaj ntau qhov chaw nqus thiab ceev ceev nqus;
3. Siv zog tsawg dua;
4. Muaj ntau qhov chaw tso nyiaj, siv tau ntau dua thiab ua tau zoo dua rau kev tsim tsiaj txhu;
5. Tshuaj tua kab mob thiab tshuaj antioxidant; 6. Kev tswj hwm lub cev tiv thaiv kab mob.
4. Kev nkag siab ntxiv txog peptides
Cov neeg siv ob hom peptide twg tau txais txiaj ntsig ntau dua?
- Kev khi peptide
- Phosphopeptide
- Cov tshuaj reagents cuam tshuam
- Cov tshuaj tua kab mob peptide
- Cov peptide tiv thaiv kab mob
- Neuropeptide
- Cov tshuaj hormones peptide
- Cov tshuaj antioxidant peptide
- Cov peptides noj haus
- Cov peptides tsw qab
(1) Kev faib tawm ntawm cov peptides
(2) Cov teebmeem ntawm lub cev ntawm cov peptides
- 1. Kho qhov sib npaug ntawm cov dej thiab electrolyte hauv lub cev;
- 2. Ua cov tshuaj tiv thaiv kab mob tiv thaiv cov kab mob thiab cov kab mob rau lub cev tiv thaiv kab mob kom txhim kho kev ua haujlwm ntawm lub cev tiv thaiv kab mob;
- 3. Txhawb kom qhov txhab kho; kho sai sai ntawm cov ntaub so ntswg epithelial raug mob.
- 4. Kev tsim cov enzymes hauv lub cev pab hloov cov khoom noj mus ua lub zog;
- 5. Kho cov hlwb, txhim kho cov metabolism hauv hlwb, tiv thaiv kev puas tsuaj ntawm cov hlwb, thiab ua lub luag haujlwm hauv kev tiv thaiv mob qog noj ntshav;
- 6. Txhawb kev tsim thiab kev tswj cov protein thiab cov enzymes;
- 7. Ib tug neeg xa xov tshuaj tseem ceeb los sib txuas lus cov ntaub ntawv ntawm cov hlwb thiab cov kabmob;
- 8. Kev tiv thaiv kab mob plawv thiab cov kab mob cerebrovascular;
- 9. Tswj cov kab mob endocrine thiab cov hlab ntsha.
- 10. Txhim kho lub cev zom zaub mov thiab kho cov kab mob plab hnyuv ntev;
- 11. Txhim kho cov kab mob ntshav qab zib, mob rheumatism, mob rheumatoid thiab lwm yam kab mob.
- 12. Tiv thaiv kab mob kis, tiv thaiv kev laus, tshem tawm cov dawb radicals ntau dhau hauv lub cev.
- 13. Txhawb kev ua haujlwm ntawm cov ntshav, kho cov ntshav tsis txaus, tiv thaiv cov ntshav sib sau ua ke, uas tuaj yeem txhim kho lub peev xwm nqa oxygen ntawm cov qe ntshav liab.
- 14. Tua ncaj qha rau cov kab mob DNA thiab tsom mus rau cov kab mob kis.
5. Kev ua haujlwm ntawm cov peptide me me chelates ob qho tib si
Tus peptide me me chelate nkag mus rau hauv lub cell tag nrho hauv lub cev tsiaj, thiabces cia li txiav cov chelation bondnyob rau hauv lub cell thiab decomposes rau hauv peptide thiab hlau ions, uas yog siv los ntawmtsiaj ua si ob txoj haujlwm noj haus, tshwj xeeb tshaj yog covpeptide ua haujlwm li cas.
Kev ua haujlwm ntawm cov peptide me me
- 1. Txhawb kom muaj protein synthesis hauv cov nqaij ntshiv ntawm cov tsiaj, txo qhov apoptosis, thiab txhawb kev loj hlob ntawm cov tsiaj
- 2. Txhim kho cov qauv ntawm cov kab mob hauv plab thiab txhawb kev noj qab haus huv ntawm plab hnyuv
- 3. Muab cov pob txha carbon thiab ua kom cov enzymes zom zaub mov zoo li plab hnyuv amylase thiab protease ua haujlwm ntau dua
- 4. Muaj cov teebmeem kev ntxhov siab tiv thaiv oxidative
- 5. Muaj cov khoom tiv thaiv kev o
- 6.……
6. Cov txiaj ntsig ntawm cov peptide me me chelates dua li cov amino acid chelates
| Cov amino acid chelated trace minerals | Cov peptide me me chelated trace minerals | |
| Tus nqi khoom siv raw | Cov khoom siv raw amino acid ib leeg kim heev | Cov khoom siv keratin raw hauv Suav teb muaj ntau heev. Cov plaub hau, cov rau tes thiab cov pob txha hauv kev yug tsiaj thiab cov dej khib nyiab protein thiab cov tawv tsiaj hauv kev lag luam tshuaj lom neeg yog cov khoom siv protein zoo thiab pheej yig. |
| Cov nyhuv nqus | Cov amino thiab carboxyl pawg koom nrog tib lub sijhawm hauv kev chelation ntawm amino acids thiab cov hlau ntsiab lus, tsim cov qauv bicyclic endocannabinoid zoo ib yam li cov dipeptides, tsis muaj cov pawg carboxyl dawb tam sim no, uas tsuas yog tuaj yeem nqus tau los ntawm oligopeptide system. (Su Chunyang et al., 2002) | Thaum cov peptides me me koom nrog hauv chelation, ib lub nplhaib chelation qauv feem ntau yog tsim los ntawm cov amino pawg kawg thiab cov pa oxygen peptide sib txuas, thiab chelate khaws cia ib pawg carboxyl dawb, uas tuaj yeem nqus tau los ntawm dipeptide system, nrog rau qhov nqus tau ntau dua li oligopeptide system. |
| Kev Ruaj Ntseg | Cov hlau ions nrog ib lossis ntau dua tsib-membered lossis rau-membered rings ntawm amino pawg, carboxyl pawg, imidazole pawg, phenol pawg, thiab sulfhydryl pawg. | Ntxiv rau tsib pawg sib koom tes uas twb muaj lawm ntawm cov amino acids, cov pawg carbonyl thiab imino hauv cov peptides me me kuj tuaj yeem koom nrog kev sib koom tes, yog li ua rau cov peptide me me chelates ruaj khov dua li cov amino acid chelates. (Yang Pin et al., 2002) |
7. Cov txiaj ntsig ntawm cov peptide me me chelates dua li glycolic acid thiab methionine chelates
| Glycine chelated trace minerals | Methionine chelated trace minerals | Cov peptide me me chelated trace minerals | |
| Daim ntawv sib koom tes | Cov carboxyl thiab amino pawg ntawm glycine tuaj yeem sib koom ua ke rau cov hlau ions. | Cov carboxyl thiab amino pawg ntawm methionine tuaj yeem sib koom ua ke rau cov hlau ions. | Thaum chelated nrog cov hlau ions, nws nplua nuj nyob rau hauv cov qauv sib koom tes thiab tsis yooj yim saturated. |
| Kev ua haujlwm ntawm kev noj haus | Cov hom thiab cov haujlwm ntawm cov amino acids yog ib qho. | Cov hom thiab cov haujlwm ntawm cov amino acids yog ib qho. | Lubntau yam nplua nujntawm cov amino acids muab kev noj haus ntau dua, thaum cov peptides me me tuaj yeem ua haujlwm raws li. |
| Cov nyhuv nqus | Glycine chelates muaj cov khoom xyaw nram qab no:nomuaj cov pab pawg carboxyl dawb thiab muaj cov nyhuv nqus qeeb. | Methionine chelates muaj cov khoom xyaw nram qab no:nomuaj cov pab pawg carboxyl dawb thiab muaj cov nyhuv nqus qeeb. | Cov peptide me me chelates tsimmuajqhov muaj cov pab pawg carboxyl dawb thiab muaj cov nyhuv nqus sai. |
Tshooj 4 Lub Npe Lag Luam "Me Peptide-mineral Chelates"
Cov Peptide-mineral Chelates me me, raws li lub npe qhia, yooj yim rau chelate.
Nws txhais tau hais tias cov peptide ligands me me, uas tsis yooj yim saturated vim muaj ntau pawg sib koom tes, Yooj yim los tsim multidentate chelate nrog cov ntsiab lus hlau, nrog kev ruaj khov zoo.
Tshooj 5 Kev Taw Qhia Txog Cov Khoom Me Me Peptide-mineral Chelates Series
1. Cov peptide me me uas muaj cov hlau nplaum chelated tooj liab (lub npe lag luam: Copper Amino Acid Chelate Feed Grade)
2. Cov peptide me me uas muaj cov hlau chelated (lub npe lag luam: Ferrous Amino Acid Chelate Feed Grade)
3. Cov peptide me me uas muaj cov minerals chelated zinc (lub npe lag luam: Zinc Amino Acid Chelate Feed Grade)
4. Cov peptide me me uas muaj cov manganese chelated (lub npe lag luam: Manganese Amino Acid Chelate Feed Grade)
Tooj liab Amino Acid Chelate Pub Qib
Ferrous Amino Acid Chelate Pub Qib
Zinc Amino Acid Chelate Pub Qib
Manganese Amino Acid Chelate Pub Qib
1. Tooj Liab Amino Acid Chelate Pub Qib
- Lub Npe Khoom: Tooj Liab Amino Acid Chelate Pub Qib
- Tsos: Cov hmoov av ntsuab
- Cov yam ntxwv ntawm lub cev thiab tshuaj lom neeg
a) Tooj liab: ≥ 10.0%
b) Tag nrho cov amino acids: ≥ 20.0%
c) Tus nqi chelation: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Cov hlau txhuas: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Cov dej noo: ≤ 5.0%
h) Kev Zoo: Tag nrho cov khoom me me dhau los ntawm 20 mesh, nrog rau qhov loj me ntawm 60-80 mesh
n = 0,1,2,... qhia txog tooj liab chelated rau dipeptides, tripeptides, thiab tetrapeptides
Diglycerin
Cov qauv ntawm cov peptide me me chelates
Cov yam ntxwv ntawm Copper Amino Acid Chelate Pub Qib
- Cov khoom no yog cov organic trace mineral chelated los ntawm cov txheej txheem tshwj xeeb chelating nrog cov nroj tsuag ntshiab enzymatic me me molecule peptides ua chelating substrates thiab trace elements.
- Cov khoom no ruaj khov hauv tshuaj lom neeg thiab tuaj yeem txo nws qhov kev puas tsuaj rau cov vitamins thiab cov rog, thiab lwm yam.
- Kev siv cov khoom no zoo rau kev txhim kho cov khoom noj kom zoo. Cov khoom no raug nqus los ntawm cov peptide me me thiab cov amino acid pathways, txo qhov kev sib tw thiab kev tawm tsam nrog lwm cov kab kawm, thiab muaj qhov zoo tshaj plaws bio-absorption thiab kev siv.
- Tooj liab yog lub ntsiab lus tseem ceeb ntawm cov qe ntshav liab, cov ntaub so ntswg sib txuas, pob txha, koom nrog lub cev ntawm ntau yam enzymes, txhim kho lub cev tiv thaiv kab mob, cov tshuaj tua kab mob, tuaj yeem ua rau qhov hnyav nce txhua hnub, txhim kho kev them nyiaj pub mis.
Kev Siv thiab Kev Ua Haujlwm ntawm Copper Amino Acid Chelate Pub Qib
| Daim ntawv thov khoom | Cov tshuaj pom zoo (g/t cov khoom muaj nqis tag nrho) | Cov ntsiab lus hauv cov khoom noj uas muaj tag nrho cov nqi (mg/kg) | Kev ua tau zoo |
| Tsob | 400 ~ 700 | 60 ~ 105 | 1. Txhim kho kev ua tau zoo ntawm kev yug me nyuam thiab kev siv xyoo ntawm cov noob taum; 2. Ua kom cov menyuam hauv plab thiab cov menyuam npua muaj zog ntxiv; 3. Txhim kho qhov kev tiv thaiv kab mob thiab kev tiv thaiv kab mob. |
| Me nyuam npua | 300 ~ 600 | 45 ~ 90 | 1. Pab tau zoo rau kev txhim kho cov ntshav hematopoietic thiab kev tiv thaiv kab mob, txhim kho kev tiv thaiv kev ntxhov siab thiab kev tiv thaiv kab mob; 2. Ua kom cov tsiaj loj hlob sai dua thiab ua kom cov tsiaj noj tau zoo dua. |
| Kev ua kom npua rog | 125 | Lub Ib Hlis 18.5 | |
| Noog | 125 | Lub Ib Hlis 18.5 | 1. Txhim kho kev tiv thaiv kev ntxhov siab thiab txo qhov tuag; 2. Txhim kho kev them nyiaj rau cov khoom noj thiab ua kom cov tsiaj loj hlob sai dua. |
| Cov tsiaj nyob hauv dej | Ntses 40 ~ 70 | 6 ~ 10.5 | 1. Txhawb kev loj hlob, txhim kho kev them nyiaj pub rau tsiaj txhu; 2. Tiv thaiv kev ntxhov siab, txo qhov mob thiab kev tuag. |
| Cws 150 ~ 200 | 22.5 ~ 30 | ||
| Tsiaj ruminant g/head hnub | Lub Ib Hlis 0.75 | 1. Tiv thaiv kom tsis txhob muaj kev hloov pauv ntawm cov pob qij txha tibial, kev txav mus los "concave back", kev co, kev puas tsuaj rau cov leeg nqaij plawv; 2. Tiv thaiv cov plaub hau lossis lub tsho tiv no keratinization, ua cov plaub hau tawv, poob qhov nkhaus ib txwm muaj, tiv thaiv kom tsis txhob muaj "cov pob grey" hauv lub voj voog qhov muag; 3. Tiv thaiv kom tsis txhob poob phaus, raws plab, thiab txo cov mis nyuj ntau lawm. |
2. Ferrous Amino Acid Chelate Pub Qib
- Lub Npe Khoom: Ferrous Amino Acid Chelate Pub Qib
- Tsos: Cov hmoov av ntsuab
- Cov yam ntxwv ntawm lub cev thiab tshuaj lom neeg
a) Hlau: ≥ 10.0%
b) Tag nrho cov amino acids: ≥ 19.0%
c) Tus nqi chelation: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Cov hlau txhuas: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Cov dej noo: ≤ 5.0%
h) Kev Zoo: Tag nrho cov khoom me me dhau los ntawm 20 mesh, nrog rau qhov loj me ntawm 60-80 mesh
n = 0,1,2,... qhia tias chelated zinc rau dipeptides, tripeptides, thiab tetrapeptides
Cov yam ntxwv ntawm Ferrous Amino Acid Chelate Pub Qib
- Cov khoom no yog cov organic trace mineral chelated los ntawm cov txheej txheem tshwj xeeb chelating nrog cov nroj tsuag ntshiab enzymatic me me molecule peptides ua chelating substrates thiab cov kab kawm;
- Cov khoom no ruaj khov hauv tshuaj lom neeg thiab tuaj yeem txo nws qhov kev puas tsuaj rau cov vitamins thiab cov rog, thiab lwm yam. Kev siv cov khoom no yog qhov zoo rau kev txhim kho cov khoom noj zoo;
- Cov khoom raug nqus los ntawm cov peptide me me thiab cov amino acid pathways, txo qhov kev sib tw thiab kev tawm tsam nrog lwm cov kab kawm, thiab muaj qhov zoo tshaj plaws bio-absorption thiab kev siv tus nqi;
- Cov khoom no tuaj yeem hla dhau qhov thaiv ntawm lub placenta thiab mammary gland, ua rau tus menyuam hauv plab noj qab haus huv zoo dua, nce qhov hnyav thaum yug los thiab qhov hnyav weaning, thiab txo qhov kev tuag; Hlau yog ib qho tseem ceeb ntawm hemoglobin thiab myoglobin, uas tuaj yeem tiv thaiv tau qhov tsis muaj hlau thiab nws cov teeb meem.
Kev Siv thiab Kev Ua Haujlwm ntawm Ferrous Amino Acid Chelate Feed Grade
| Daim ntawv thov khoom | Cov tshuaj pom zoo (g/t tag nrho cov khoom muaj nqis) | Cov ntsiab lus hauv cov khoom noj uas muaj tag nrho cov nqi (mg/kg) | Kev ua tau zoo |
| Tsob | 300 ~ 800 | 45 ~ 120 | 1. Txhim kho kev ua tau zoo ntawm kev yug me nyuam thiab kev siv lub neej ntawm cov npua; 2. txhim kho qhov hnyav thaum yug los, qhov hnyav thaum tsis noj mis thiab qhov sib npaug ntawm cov menyuam npua kom ua tau zoo dua hauv lub sijhawm tom qab; 3. Txhim kho qhov khaws cia hlau hauv cov npua uas pub niam mis thiab qhov muaj hlau ntau hauv mis kom tiv thaiv kev tsis muaj hlau hauv cov npua uas pub niam mis. |
| Cov npua me thiab cov npua rog | Cov menyuam npua 300 ~ 600 | 45 ~ 90 | 1. Txhim kho kev tiv thaiv kab mob ntawm cov menyuam npua, txhim kho kev tiv thaiv kab mob thiab txhim kho tus nqi ciaj sia; 2. Ua kom cov npua loj hlob sai dua, txhim kho kev hloov pauv zaub mov, ua kom cov quav qaib hnyav dua thiab sib npaug, thiab txo qhov muaj cov npua mob; 3. Txhim kho myoglobin thiab myoglobin theem, tiv thaiv thiab kho cov ntshav tsis txaus hlau, ua rau daim tawv nqaij npua liab thiab pom tseeb tias xim nqaij zoo dua. |
| Ua kom npua rog 200 ~ 400 | 30 ~ 60 | ||
| Noog | 300 ~ 400 | 45 ~ 60 | 1. Txhim kho kev hloov pauv pub mis, ua kom muaj kev loj hlob sai, txhim kho kev muaj peev xwm tiv thaiv kev ntxhov siab thiab txo qhov tuag; 2. Txhim kho qhov kev tso qe, txo qhov kev tawg ntawm cov qe thiab ua kom cov xim ntawm cov qe daj tob dua; 3. Txhim kho qhov kev loj hlob ntawm cov qe thiab cov qe uas yuav yug me nyuam thiab qhov kev ciaj sia ntawm cov qaib hluas. |
| Cov tsiaj nyob hauv dej | 200 ~ 300 | 30 ~ 45 | 1. Txhawb kev loj hlob, txhim kho kev hloov pauv pub; 2. Txhim kho kev tiv thaiv kev ntxhov siab, txo kev mob nkeeg thiab kev tuag. |
3. Zinc Amino Acid Chelate Pub Qib
- Lub Npe Khoom: Zinc Amino Acid Chelate Pub Qib
- Tsos: granules xim av daj
- Cov yam ntxwv ntawm lub cev thiab tshuaj lom neeg
a) Zinc: ≥ 10.0%
b) Tag nrho cov amino acids: ≥ 20.5%
c) Tus nqi chelation: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Cov hlau txhuas: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Cov dej noo: ≤ 5.0%
h) Kev Zoo: Tag nrho cov khoom me me dhau los ntawm 20 mesh, nrog rau qhov loj me ntawm 60-80 mesh
n = 0,1,2,... qhia tias chelated zinc rau dipeptides, tripeptides, thiab tetrapeptides
Cov yam ntxwv ntawm Zinc Amino Acid Chelate Pub Qib
Cov khoom no yog cov organic trace mineral chelated los ntawm cov txheej txheem tshwj xeeb chelating nrog cov nroj tsuag ntshiab enzymatic me me molecule peptides ua chelating substrates thiab cov kab kawm;
Cov khoom no ruaj khov hauv tshuaj lom neeg thiab tuaj yeem txo nws qhov kev puas tsuaj rau cov vitamins thiab cov rog, thiab lwm yam.
Kev siv cov khoom no yog qhov zoo rau kev txhim kho cov khoom noj zoo; Cov khoom raug nqus los ntawm cov peptide me me thiab cov amino acid pathways, txo qhov kev sib tw thiab kev tawm tsam nrog lwm cov kab kawm, thiab muaj qhov zoo tshaj plaws bio-absorption thiab kev siv tus nqi;
Cov khoom no tuaj yeem txhim kho kev tiv thaiv kab mob, txhawb kev loj hlob, ua rau muaj kev hloov pauv pub mis ntau ntxiv thiab txhim kho cov plaub hau ci ntsa iab;
Zinc yog ib qho tseem ceeb ntawm ntau tshaj 200 cov enzymes, epithelial tissue, ribose thiab gustatin. Nws txhawb kom cov qe ntshav saj hauv tus nplaig mucosa loj hlob sai thiab tswj kev ntshaw; tiv thaiv cov kab mob hauv plab hnyuv; thiab muaj cov haujlwm ua tshuaj tua kab mob, uas tuaj yeem txhim kho kev ua haujlwm ntawm lub cev zom zaub mov thiab kev ua haujlwm ntawm cov enzymes hauv cov nqaij thiab cov hlwb.
Kev Siv thiab Kev Ua Haujlwm ntawm Zinc Amino Acid Chelate Pub Qib
| Daim ntawv thov khoom | Cov tshuaj pom zoo (g/t tag nrho cov khoom muaj nqis) | Cov ntsiab lus hauv cov khoom noj uas muaj tag nrho cov nqi (mg/kg) | Kev ua tau zoo |
| Cov poj niam cev xeeb tub thiab cov poj niam uas tab tom pub niam mis | 300 ~ 500 | 45 ~ 75 | 1. Txhim kho kev ua tau zoo ntawm kev yug me nyuam thiab kev siv lub neej ntawm cov npua; 2. Txhim kho lub zog ntawm tus menyuam hauv plab thiab cov menyuam npua, txhim kho kev tiv thaiv kab mob, thiab ua rau lawv muaj kev ua tau zoo dua hauv theem tom qab; 3. Txhim kho lub cev nqaij daim tawv ntawm cov npua cev xeeb tub thiab qhov hnyav ntawm cov npua yug los. |
| Menyuam npua haus luam yeeb, menyuam npua thiab cov npua uas tab tom loj hlob rog | 250 ~ 400 | 37.5 ~ 60 | 1. Txhim kho kev tiv thaiv kab mob ntawm cov menyuam npua, txo qhov raws plab thiab kev tuag; 2. Txhim kho qhov saj, ua kom cov zaub mov noj ntau ntxiv, ua kom cov tsiaj loj hlob sai dua thiab txhim kho kev hloov pauv zaub mov; 3. Ua kom lub tsho npua ci ntsa iab thiab txhim kho qhov zoo ntawm lub cev thiab cov nqaij. |
| Noog | 300 ~ 400 | 45 ~ 60 | 1. Txhim kho cov plaub glossiness; 2. txhim kho tus nqi tso, tus nqi fertilization thiab tus nqi hatching ntawm cov qe yug me nyuam, thiab txhawb kom muaj peev xwm xim ntawm cov qe yolk; 3. Txhim kho kev muaj peev xwm tiv thaiv kev ntxhov siab thiab txo qhov tuag taus; 4. Txhim kho kev hloov pauv pub thiab ua kom muaj kev loj hlob sai. |
| Cov tsiaj nyob hauv dej | Lub Ib Hlis 300 | 45 | 1. Txhawb kev loj hlob, txhim kho kev hloov pauv pub; 2. Txhim kho kev tiv thaiv kev ntxhov siab, txo kev mob nkeeg thiab kev tuag. |
| Tsiaj ruminant g/head hnub | 2.4 | 1. Txhim kho cov mis nyuj kom zoo, tiv thaiv mob mastitis thiab kab mob lwj, thiab txo cov hlwb somatic hauv mis nyuj; 2. Txhawb kev loj hlob, txhim kho kev hloov pauv pub thiab txhim kho cov nqaij zoo. |
4. Manganese Amino Acid Chelate Pub Qib
- Lub Npe Khoom: Manganese Amino Acid Chelate Pub Qib
- Tsos: granules xim av daj
- Cov yam ntxwv ntawm lub cev thiab tshuaj lom neeg
a) Mn: ≥ 10.0%
b) Tag nrho cov amino acids: ≥ 19.5%
c) Tus nqi chelation: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Cov hlau txhuas: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Cov dej noo: ≤ 5.0%
h) Kev Zoo: Tag nrho cov khoom me me dhau los ntawm 20 mesh, nrog rau qhov loj me ntawm 60-80 mesh
n = 0, 1,2, ... qhia tias chelated manganese rau dipeptides, tripeptides, thiab tetrapeptides
Cov yam ntxwv ntawm Manganese Amino Acid Chelate Pub Qib
Cov khoom no yog cov organic trace mineral chelated los ntawm cov txheej txheem tshwj xeeb chelating nrog cov nroj tsuag ntshiab enzymatic me me molecule peptides ua chelating substrates thiab cov kab kawm;
Cov khoom no ruaj khov hauv tshuaj lom neeg thiab tuaj yeem txo nws qhov kev puas tsuaj rau cov vitamins thiab cov rog, thiab lwm yam. Kev siv cov khoom no yog qhov zoo rau kev txhim kho cov khoom noj zoo;
Cov khoom raug nqus los ntawm cov peptide me me thiab cov amino acid pathways, txo qhov kev sib tw thiab kev tawm tsam nrog lwm cov kab kawm, thiab muaj qhov zoo tshaj plaws bio-absorption thiab kev siv tus nqi;
Cov khoom no tuaj yeem txhim kho qhov kev loj hlob, txhim kho kev hloov pauv zaub mov thiab kev noj qab haus huv zoo heev; thiab txhim kho qhov kev tso qe, qhov kev tso qe thiab qhov kev noj qab haus huv ntawm cov qaib yug me nyuam kom pom tseeb;
Manganese yog qhov tsim nyog rau kev loj hlob ntawm pob txha thiab kev kho cov ntaub so ntswg sib txuas. Nws muaj feem cuam tshuam nrog ntau yam enzymes; thiab koom nrog hauv kev ua kom cov carbohydrate, rog thiab protein metabolism, kev yug me nyuam thiab kev tiv thaiv kab mob.
Kev Siv thiab Kev Ua Haujlwm ntawm Manganese Amino Acid Chelate Pub Qib
| Daim ntawv thov khoom | Cov tshuaj pom zoo (g/t cov khoom muaj nqis tag nrho) | Cov ntsiab lus hauv cov khoom noj uas muaj tag nrho cov nqi (mg/kg) | Kev ua tau zoo |
| Kev yug npua | 200 ~ 300 | 30 ~ 45 | 1. Txhawb kom muaj kev loj hlob ntawm cov kab mob sib deev thiab txhim kho kev txav ntawm cov phev; 2. Txhim kho lub peev xwm yug me nyuam ntawm cov npua yug me nyuam thiab txo cov teeb meem kev yug me nyuam. |
| Cov npua me thiab cov npua rog | 100 ~ 250 | 15 ~ 37.5 | 1. Nws muaj txiaj ntsig zoo rau kev txhim kho kev tiv thaiv kab mob, thiab txhim kho kev tiv thaiv kev ntxhov siab thiab kev tiv thaiv kab mob; 2. Txhawb kev loj hlob thiab txhim kho kev hloov pauv pub zaub mov kom zoo; 3. Txhim kho cov xim nqaij thiab qhov zoo, thiab txhim kho feem pua ntawm cov nqaij ntshiv. |
| Noog | 250 ~ 350 | 37.5 ~ 52.5 | 1. Txhim kho kev muaj peev xwm tiv thaiv kev ntxhov siab thiab txo qhov tuag taus; 2. Txhim kho tus nqi tso, tus nqi fertilization thiab tus nqi hatching ntawm cov qe yug me nyuam, txhim kho qhov zoo ntawm lub plhaub qe thiab txo qhov kev tawg ntawm lub plhaub; 3. Txhawb kev loj hlob ntawm pob txha thiab txo qhov tshwm sim ntawm cov kab mob ceg. |
| Cov tsiaj nyob hauv dej | 100 ~ 200 | 15 ~ 30 | 1. Txhawb kev loj hlob thiab txhim kho nws lub peev xwm tiv thaiv kev ntxhov siab thiab kev tiv thaiv kab mob; 2. Txhim kho kev txav ntawm cov phev thiab kev tawm ntawm cov qe uas tau cog qoob loo. |
| Tsiaj ruminant g/head hnub | Nyuj 1.25 | 1. Tiv thaiv kev tsim cov roj fatty acid thiab kev puas tsuaj rau cov pob txha; 2. Txhim kho lub peev xwm yug me nyuam, tiv thaiv kev rho menyuam thiab kev tuag tes tuag taw tom qab yug me nyuam ntawm cov tsiaj poj niam, txo qhov tuag ntawm cov menyuam nyuj thiab cov menyuam yaj, thiab ua rau cov tsiaj hluas hnyav dua. | |
| Tshis 0.25 |
Tshooj 6 FAB ntawm Cov Peptide-mineral Chelates Me Me
| S/N | F: Cov yam ntxwv ua haujlwm | A: Kev sib tw sib txawv | B: Cov txiaj ntsig los ntawm kev sib tw sib txawv rau cov neeg siv |
| 1 | Kev tswj hwm kev xaiv ntawm cov khoom siv raw | Xaiv cov nroj tsuag ntshiab enzymatic hydrolysis ntawm cov peptides me me | Kev nyab xeeb ntawm lub cev muaj zog, zam kev noj neeg |
| 2 | Kev siv tshuab zom zaub mov rau ob hom protein biological enzyme | Feem pua ntawm cov peptides me me | Ntau "lub hom phiaj", uas tsis yooj yim rau kev saturation, nrog rau kev ua haujlwm siab ntawm lub cev thiab kev ruaj khov zoo dua |
| 3 | Kev siv tshuab txau thiab ziab siab heev | Cov khoom granular, nrog rau qhov loj me me, zoo dua fluidity, tsis yooj yim rau nqus dej noo | Xyuas kom yooj yim siv, sib tov sib npaug ntau dua hauv cov khoom noj tag nrho |
| Cov dej tsawg (≤ 5%), uas txo qhov cuam tshuam los ntawm cov vitamins thiab cov enzyme npaj | Txhim kho qhov ruaj khov ntawm cov khoom noj pub | ||
| 4 | Kev siv tshuab tswj kev tsim khoom siab heev | Cov txheej txheem kaw tag nrho, qib siab ntawm kev tswj hwm tsis siv neeg | Muaj kev nyab xeeb thiab ruaj khov zoo |
| 5 | Kev siv tshuab tswj xyuas zoo heev | Tsim thiab txhim kho cov txheej txheem kev tshuaj xyuas kev tshawb fawb thiab kev tshuaj xyuas siab heev thiab cov cuab yeej tswj hwm rau kev nrhiav cov yam ntxwv cuam tshuam rau qhov zoo ntawm cov khoom, xws li cov protein uas yaj tau hauv cov kua qaub, kev faib tawm ntawm qhov hnyav ntawm cov molecular, cov amino acids thiab tus nqi chelating. | Xyuas kom zoo, xyuas kom meej thiab txhim kho kev ua haujlwm zoo |
Tshooj 7 Kev Sib Piv ntawm Cov Neeg Sib Tw
Tus Qauv VS Tus Qauv
Kev sib piv ntawm peptide faib thiab chelation tus nqi ntawm cov khoom
| Cov khoom ntawm Sustar | Feem pua ntawm cov peptides me me (180-500) | Cov khoom ntawm Zinpro | Feem pua ntawm cov peptides me me (180-500) |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | MUAB TAU-Fe | 59% |
| AA-Mn | ≥33% | MAVILA-Mn | 53% |
| AA-Zn | ≥37% | MUAB TAU-Zn | 56% |
| Cov khoom ntawm Sustar | Tus nqi chelation | Cov khoom ntawm Zinpro | Tus nqi chelation |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | MUAB TAU-Fe | 93.5% |
| AA-Mn | 94.6% | MAVILA-Mn | 94.6% |
| AA-Zn | 97.7% | MUAB TAU-Zn | 90.6% |
Qhov piv ntawm cov peptides me me ntawm Sustar qis dua me ntsis ntawm Zinpro, thiab tus nqi chelation ntawm Sustar cov khoom yog siab dua me ntsis ntawm Zinpro cov khoom.
Kev sib piv ntawm cov ntsiab lus ntawm 17 amino acids hauv cov khoom sib txawv
| Lub npe ntawm cov amino acids | Sustar's Copper Amino Acid Chelate Qib pub | Zinpro's MUAB TAU tooj liab | Sustar's Ferrous Amino Acid C Cov khoom noj helate Qib | Zinpro muaj khoom hlau | Sustar's Manganese Amino Acid Chelate Qib pub | Zinpro muaj khoom manganese | Zinc ntawm Sustar Cov kua qaub amino Chelate Pub Qib | Zinpro muaj khoom zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | Lub Ib Hlis 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Tag nrho cov amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Zuag qhia tag nrho, qhov feem pua ntawm cov amino acids hauv Sustar cov khoom yog siab dua li ntawm Zinpro cov khoom.
Tshooj 8 Cov teebmeem ntawm kev siv
Cov teebmeem ntawm ntau qhov chaw sib txawv ntawm cov zaub mov me me rau kev ua tau zoo thiab qe zoo ntawm cov qaib tso thaum lub sijhawm tso qe lig
Cov Txheej Txheem Tsim Khoom
- Kev siv tshuab chelation tsom mus rau
- Kev siv tshuab emulsification shear
- Kev siv tshuab txau thiab ziab siab
- Kev siv tshuab ua kom txias thiab tshem dej noo
- Kev siv tshuab tswj ib puag ncig siab heev
Cov Lus Qhia Ntxiv A: Cov Txheej Txheem rau Kev Txheeb Xyuas Qhov Kev Faib Tawm ntawm Cov Piam Thaj ntawm Cov Peptides
Kev txais yuav tus qauv: GB / T 22492-2008
1 Lub Ntsiab Cai ntawm Kev Ntsuas:
Nws tau txiav txim siab los ntawm kev siv high-performance gel filtration chromatography. Uas yog hais tias, siv cov khoom siv porous ua theem ruaj khov, raws li qhov sib txawv ntawm qhov loj me ntawm cov khoom siv piv txwv rau kev sib cais, pom ntawm peptide bond ntawm ultraviolet absorption wavelength ntawm 220 nm, siv cov software ua cov ntaub ntawv tshwj xeeb rau kev txiav txim siab ntawm qhov faib tawm ntawm cov khoom siv molecular los ntawm gel filtration chromatography (piv txwv li, GPC software), cov chromatograms thiab lawv cov ntaub ntawv tau ua tiav, xam kom tau txais qhov loj me ntawm qhov sib piv ntawm cov peptide soybean thiab qhov faib tawm.
2. Cov tshuaj reagents
Cov dej sim yuav tsum ua tau raws li cov lus qhia ntawm cov dej theem nrab hauv GB / T6682, kev siv cov tshuaj reagents, tshwj tsis yog cov kev cai tshwj xeeb, yog qhov ntshiab analytically.
2.1 Cov tshuaj reagents suav nrog acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Cov tshuaj txheem siv rau hauv qhov nkhaus calibration ntawm qhov faib tawm ntawm cov molecular mass: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Cov Cuab Yeej thiab cov khoom siv
3.1 High Performance Liquid Chromatograph (HPLC): ib lub chaw ua haujlwm chromatographic lossis integrator nrog lub UV detector thiab GPC data processing software.
3.2 Lub tshuab nqus tsev thiab lim dej ntawm theem txawb.
3.3 Lub tshuab hluav taws xob sib npaug: tus nqi kawm tiav 0.000 1g.
4 Cov kauj ruam ua haujlwm
4.1 Cov xwm txheej Chromatographic thiab kev sim hloov kho lub kaw lus (cov xwm txheej siv)
4.1.1 Chromatographic kem: TSKgelG2000swxl300 mm × 7.8 mm (sab hauv txoj kab uas hla) lossis lwm cov gel kem ntawm tib hom nrog kev ua tau zoo sib xws uas tsim nyog rau kev txiav txim siab ntawm cov protein thiab peptides.
4.1.2 Txawb theem: Acetonitrile + dej + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Qhov ntev ntawm kev kuaj pom: 220 nm.
4.1.4 Tus nqi ntws: 0.5 mL / feeb.
4.1.5 Lub sijhawm nrhiav pom: 30 feeb.
4.1.6 Qhov ntim ntawm cov qauv txhaj tshuaj: 20μL.
4.1.7 Kub ntawm kem: kub hauv chav.
4.1.8 Yuav kom ua rau lub kaw lus chromatographic ua tau raws li qhov yuav tsum tau ua kom pom tseeb, nws tau teev tseg tias nyob rau hauv cov xwm txheej chromatographic saum toj no, qhov ua tau zoo ntawm cov kem chromatographic gel, piv txwv li, tus lej theoretical ntawm cov phaj (N), tsis tsawg dua 10000 xam raws li cov ncov ntawm tus qauv tripeptide (Glycine-Glycine-Glycine).
4.2 Kev tsim cov qauv ntsuas molecular mass
Cov qauv sib txawv ntawm cov qauv molecular mass peptide uas muaj qhov sib txawv ntawm 1 mg / mL tau npaj los ntawm kev sib phim theem txawb, sib xyaw ua ke hauv qee qhov sib piv, thiab tom qab ntawd lim los ntawm cov organic theem membrane nrog qhov loj ntawm 0.2 μm ~ 0.5 μm thiab txhaj rau hauv cov qauv, thiab tom qab ntawd cov chromatograms ntawm cov qauv tau txais. Cov kab sib npaug ntawm cov qauv molecular calibration thiab lawv cov kab zauv tau txais los ntawm kev plotting logarithm ntawm cov qauv molecular sib piv tiv thaiv lub sijhawm khaws cia lossis los ntawm linear regression.
4.3 Kev kho cov qauv
Muab 10mg ntawm cov qauv ntsuas rau hauv lub raj mis volumetric 10mL, ntxiv me ntsis mobile phase, thiab co ultrasonic rau 10 feeb, kom cov qauv yaj tag thiab sib xyaw, diluted nrog mobile phase rau ntawm qhov ntsuas, thiab tom qab ntawd lim los ntawm daim nyias nyias organic phase nrog qhov loj ntawm 0.2μm ~ 0.5μm, thiab cov lim dej tau soj ntsuam raws li cov xwm txheej chromatographic hauv A.4.1.
5. Kev suav ntawm kev faib tawm ntawm cov pawg molecular sib piv
Tom qab tshuaj xyuas cov kua qauv uas tau npaj tseg hauv 4.3 nyob rau hauv cov xwm txheej chromatographic ntawm 4.1, qhov hnyav molecular ntawm cov qauv thiab nws qhov kev faib tawm tuaj yeem tau txais los ntawm kev hloov cov ntaub ntawv chromatographic ntawm cov qauv rau hauv qhov nkhaus calibration 4.2 nrog GPC cov ntaub ntawv ua software. Kev faib tawm ntawm cov hnyav molecular ntawm cov peptides sib txawv tuaj yeem suav los ntawm txoj kev ua kom thaj chaw siab tshaj plaws, raws li cov mis: X = A / A tag nrho × 100
Hauv cov mis: X - Qhov feem pua ntawm cov molecular mass peptide hauv tag nrho cov peptide hauv cov qauv,%;
A - Thaj chaw siab tshaj plaws ntawm cov peptide molecular mass;
Tag Nrho A - qhov sib sau ua ke ntawm cov cheeb tsam siab tshaj plaws ntawm txhua tus peptide molecular mass, suav rau ib qho chaw decimal.
6 Rov ua dua
Qhov sib txawv kiag li ntawm ob qhov kev txiav txim siab ywj pheej uas tau txais raws li cov xwm txheej ntawm kev rov ua dua yuav tsum tsis pub tshaj 15% ntawm qhov nruab nrab ntawm kev suav lej ntawm ob qhov kev txiav txim siab.
Cov Lus Qhia Ntxiv B: Cov Txheej Txheem rau Kev Txheeb Xyuas Cov Amino Acids Dawb
Kev txais yuav tus qauv: Q/320205 KAVN05-2016
1.2 Cov tshuaj reagents thiab cov ntaub ntawv
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Qhov Qhia: 0.1% crystal violet indicator (glacial acetic acid)
2. Kev txiav txim siab ntawm cov amino acids dawb
Cov qauv tau qhuav ntawm 80 ° C rau 1 teev.
Muab cov qauv tso rau hauv lub thawv qhuav kom txias mus rau qhov kub hauv chav lossis txias mus rau qhov kub uas siv tau.
Hnyav kwv yees li 0.1 g ntawm cov qauv (qhov tseeb txog 0.001 g) rau hauv lub raj mis 250 mL qhuav conical.
Ua sai sai mus rau kauj ruam tom ntej kom tsis txhob muaj cov qauv nqus cov dej noo ib puag ncig
Ntxiv 25 mL ntawm glacial acetic acid thiab sib tov kom zoo tsis pub tshaj 5 feeb.
Ntxiv 2 tee ntawm cov xim crystal violet indicator
Titrate nrog 0.0500 mol / L (± 0.001) tus qauv titration kua ntawm perchloric acid kom txog thaum cov kua hloov ntawm xim av mus rau qhov kawg.
Sau qhov ntim ntawm cov kua txheem uas tau noj.
Ua qhov kev xeem dawb paug tib lub sijhawm.
3. Kev suav thiab cov txiaj ntsig
Cov amino acid dawb uas muaj nyob hauv X hauv cov reagent yog qhia ua feem pua (%) thiab suav raws li cov mis: X = C × (V1-V0) × 0.1445/M × 100%, hauv cov mis tne:
C - Kev sib xyaw ntawm cov kua qaub perchloric acid hauv moles ib liter (mol/L)
V1 - Ntim siv rau titration ntawm cov qauv nrog tus qauv perchloric acid kua, hauv milliliters (mL).
Vo - Ntim siv rau titration dawb nrog tus qauv perchloric acid kua, hauv milliliters (mL);
M - Qhov hnyav ntawm cov qauv, hauv grams (g).
0.1445: Qhov hnyav nruab nrab ntawm cov amino acids sib npaug rau 1.00 mL ntawm cov kua qaub perchloric acid txheem [c (HClO4) = 1.000 mol / L].
Cov Lus Qhia Ntxiv C: Cov Txheej Txheem rau Kev Txheeb Xyuas Tus Nqi Chelation ntawm Sustar
Kev txais yuav cov qauv: Q/70920556 71-2024
1. Txoj cai txiav txim siab (Fe ua piv txwv)
Cov amino acid hlau complexes muaj solubility tsawg heev hauv anhydrous ethanol thiab cov hlau ions dawb yog soluble hauv anhydrous ethanol, qhov sib txawv ntawm solubility ntawm ob qho hauv anhydrous ethanol tau siv los txiav txim siab qhov chelation rate ntawm amino acid hlau complexes.
2. Cov Tshuaj Reagents & Cov Tshuaj
Anhydrous ethanol; tus so yog tib yam li kab lus 4.5.2 hauv GB / T 27983-2011.
3. Cov kauj ruam ntawm kev tshuaj xyuas
Ua ob qhov kev sim ua ke. Hnyav 0.1g ntawm cov qauv qhuav ntawm 103 ± 2 ℃ rau 1 teev, raug rau 0.0001g, ntxiv 100mL ntawm anhydrous ethanol kom yaj, lim, lim cov seem ntxuav nrog 100mL ntawm anhydrous ethanol tsawg kawg peb zaug, tom qab ntawd hloov cov seem rau hauv lub raj mis conical 250mL, ntxiv 10mL ntawm sulfuric acid kua raws li kab lus 4.5.3 hauv GB / T27983-2011, thiab tom qab ntawd ua cov kauj ruam hauv qab no raws li kab lus 4.5.3 "Sov kom yaj thiab tom qab ntawd cia txias" hauv GB / T27983-2011. Ua qhov kev sim dawb paug tib lub sijhawm.
4. Kev txiav txim siab ntawm tag nrho cov ntsiab lus hlau
4.1 Lub hauv paus ntsiab lus ntawm kev txiav txim siab zoo ib yam li kab lus 4.4.1 hauv GB / T 21996-2008.
4.2. Cov Tshuaj Reagents & Cov Tshuaj
4.2.1 Cov kua qaub sib xyaw: Ntxiv 150mL ntawm cov kua qaub sulfuric thiab 150mL ntawm cov kua qaub phosphoric rau hauv 700mL dej thiab sib tov kom zoo.
4.2.2 Sodium diphenylamine sulfonate qhia txog kev daws teeb meem: 5g / L, npaj raws li GB / T603.
4.2.3 Cerium sulfate tus qauv titration kua: concentration c [Ce (SO4) 2] = 0.1 mol/L, npaj raws li GB/T601.
4.3 Cov Kauj Ruam ntawm Kev Tshuaj Xyuas
Ua ob qho kev sim ua ke. Hnyav 0.1g ntawm cov qauv, raug rau 020001g, muab tso rau hauv lub raj mis conical 250mL, ntxiv 10mL ntawm cov kua qaub sib xyaw, tom qab yaj, ntxiv 30ml dej thiab 4 tee ntawm sodium dianiline sulfonate qhia cov kua, thiab tom qab ntawd ua cov kauj ruam hauv qab no raws li kab lus 4.4.2 hauv GB / T21996-2008. Ua qhov kev sim dawb paug tib lub sijhawm.
4.4 Kev sawv cev ntawm cov txiaj ntsig
Tag nrho cov hlau ntsiab lus X1 ntawm cov amino acid hlau complexes nyob rau hauv cov nqe lus ntawm pawg loj ntawm hlau, tus nqi qhia nyob rau hauv%, tau suav raws li cov mis (1):
X1=(V-V0)×C×M×10-3×100
Hauv cov mis: V - ntim ntawm cerium sulfate tus qauv kua siv rau titration ntawm kev sim kua, mL;
V0 - cerium sulfate tus qauv kua siv rau titration ntawm cov kua dawb paug, mL;
C - Qhov tseeb concentration ntawm cerium sulfate txheem kua, mol/L
5. Kev suav cov ntsiab lus hlau hauv chelates
Cov ntsiab lus hlau X2 hauv chelate hais txog qhov feem pua ntawm cov hlau, tus nqi qhia hauv%, tau suav raws li cov mis: x2 = ((V1-V2) × C × 0.05585) / m1 × 100
Hauv cov mis: V1 - ntim ntawm cerium sulfate tus qauv kua siv rau titration ntawm kev sim kua, mL;
V2 - cerium sulfate tus qauv kua siv rau titration ntawm cov kua dawb paug, mL;
C - Qhov concentration tiag tiag ntawm cerium sulfate standard solution, mol/L;
0.05585 - qhov hnyav ntawm cov hlau ferrous qhia hauv grams sib npaug rau 1.00 mL ntawm cerium sulfate txheem kua C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Qhov hnyav ntawm cov qauv, g. Siv qhov nruab nrab ntawm cov txiaj ntsig kev txiav txim siab sib luag ua cov txiaj ntsig kev txiav txim siab, thiab qhov sib txawv kiag li ntawm cov txiaj ntsig kev txiav txim siab sib luag tsis pub ntau tshaj 0.3%.
6. Kev suav tus nqi chelation
Tus nqi chelation X3, tus nqi qhia hauv %, X3 = X2/X1 × 100
Cov Lus Qhia Ntxiv C: Cov Txheej Txheem rau Kev Txheeb Xyuas Zinpro's chelation rate
Kev txais yuav tus qauv: Q/320205 KAVNO7-2016
1. Cov tshuaj reagents thiab cov ntaub ntawv
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Qhov Ntsuas: 0.1% crystal violet indicator (glacial acetic acid)
2. Kev txiav txim siab ntawm cov amino acids dawb
2.1 Cov qauv tau qhuav ntawm 80 ° C rau 1 teev.
2.2 Muab cov qauv tso rau hauv lub thawv qhuav kom txias mus rau qhov kub hauv chav lossis txias mus rau qhov kub uas siv tau.
2.3 Hnyav kwv yees li 0.1 g ntawm cov qauv (qhov tseeb txog 0.001 g) rau hauv lub raj mis qhuav 250 mL conical
2.4 Ua sai sai mus rau kauj ruam tom ntej kom tsis txhob muaj cov qauv nqus cov dej noo ib puag ncig.
2.5 Ntxiv 25mL ntawm glacial acetic acid thiab sib tov kom zoo tsis pub dhau 5 feeb.
2.6 Ntxiv 2 tee ntawm cov xim qhia xim liab dawb.
2.7 Titrate nrog 0.0500mol/L (±0.001) tus qauv titration kua ntawm perchloric acid kom txog thaum cov kua hloov ntawm xim av mus rau ntsuab rau 15 vib nas this yam tsis hloov xim ua qhov kawg.
2.8 Sau qhov ntim ntawm cov kua dej ib txwm siv.
2.9 Ua qhov kev xeem dawb paug tib lub sijhawm.
3. Kev suav thiab cov txiaj ntsig
Cov amino acid dawb uas muaj nyob hauv cov reagent yog qhia ua feem pua (%), xam raws li cov mis (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
Hauv cov mis: C - qhov concentration ntawm cov kua qaub perchloric acid txheem hauv moles ib liter (mol/L)
V1 - Ntim siv rau titration ntawm cov qauv nrog tus qauv perchloric acid kua, hauv milliliters (mL).
Vo - Ntim siv rau titration dawb nrog tus qauv perchloric acid kua, hauv milliliters (mL);
M - Qhov hnyav ntawm cov qauv, hauv grams (g).
0.1445 - Qhov hnyav nruab nrab ntawm cov amino acids sib npaug rau 1.00 mL ntawm cov kua qaub perchloric acid txheem [c (HClO4) = 1.000 mol / L].
4. Kev suav tus nqi chelation
Tus nqi chelation ntawm cov qauv yog qhia ua pawg loj (%), xam raws li cov qauv (2): tus nqi chelation = (tag nrho cov amino acid cov ntsiab lus - cov amino acid dawb cov ntsiab lus) / tag nrho cov amino acid cov ntsiab lus × 100%.
Lub sijhawm tshaj tawm: Cuaj hlis-17-2025
