2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Cov tshuaj txheem siv rau hauv qhov nkhaus calibration ntawm qhov faib tawm ntawm cov molecular mass: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Cov Cuab Yeej thiab cov khoom siv
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Zuag qhia tag nrho, qhov feem pua ntawm cov amino acids hauv Sustar cov khoom yog siab dua li ntawm Zinpro cov khoom.
Tshooj 8 Cov teebmeem ntawm kev siv
Cov teebmeem ntawm ntau qhov chaw sib txawv ntawm cov zaub mov me me rau kev ua tau zoo thiab qe zoo ntawm cov qaib tso thaum lub sijhawm tso qe lig
Cov Txheej Txheem Tsim Khoom
Kev siv tshuab chelation tsom mus rau
Kev siv tshuab emulsification shear
Kev siv tshuab txau thiab ziab siab
Kev siv tshuab ua kom txias thiab tshem dej noo
Kev siv tshuab tswj ib puag ncig siab heev
Cov Lus Qhia Ntxiv A: Cov Txheej Txheem rau Kev Txheeb Xyuas Qhov Kev Faib Tawm ntawm Cov Piam Thaj ntawm Cov Peptides
Kev txais yuav tus qauv: GB / T 22492-2008
1 Lub Ntsiab Cai ntawm Kev Ntsuas:
Nws tau txiav txim siab los ntawm kev siv high-performance gel filtration chromatography. Uas yog hais tias, siv cov khoom siv porous ua theem ruaj khov, raws li qhov sib txawv ntawm qhov loj me ntawm cov khoom siv piv txwv rau kev sib cais, pom ntawm peptide bond ntawm ultraviolet absorption wavelength ntawm 220 nm, siv cov software ua cov ntaub ntawv tshwj xeeb rau kev txiav txim siab ntawm qhov faib tawm ntawm cov khoom siv molecular los ntawm gel filtration chromatography (piv txwv li, GPC software), cov chromatograms thiab lawv cov ntaub ntawv tau ua tiav, xam kom tau txais qhov loj me ntawm qhov sib piv ntawm cov peptide soybean thiab qhov faib tawm.
2. Cov tshuaj reagents
Cov dej sim yuav tsum ua tau raws li cov lus qhia ntawm cov dej theem nrab hauv GB / T6682, kev siv cov tshuaj reagents, tshwj tsis yog cov kev cai tshwj xeeb, yog qhov ntshiab analytically.
2.1 Cov tshuaj reagents suav nrog acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Cov tshuaj txheem siv rau hauv qhov nkhaus calibration ntawm qhov faib tawm ntawm cov molecular mass: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Cov Cuab Yeej thiab cov khoom siv
3.1 High Performance Liquid Chromatograph (HPLC): ib lub chaw ua haujlwm chromatographic lossis integrator nrog lub UV detector thiab GPC data processing software.
3.2 Lub tshuab nqus tsev thiab lim dej ntawm theem txawb.
3.3 Lub tshuab hluav taws xob sib npaug: tus nqi kawm tiav 0.000 1g.
4 Cov kauj ruam ua haujlwm
4.1 Cov xwm txheej Chromatographic thiab kev sim hloov kho lub kaw lus (cov xwm txheej siv)
- 4.1.1 Chromatographic kem: TSKgelG2000swxl300 mm × 7.8 mm (sab hauv txoj kab uas hla) lossis lwm cov gel kem ntawm tib hom nrog kev ua tau zoo sib xws uas tsim nyog rau kev txiav txim siab ntawm cov protein thiab peptides.
- 4.1.2 Txawb theem: Acetonitrile + dej + trifluoroacetic acid = 20 + 80 + 0.1.
- 4.1.3 Qhov ntev ntawm kev kuaj pom: 220 nm.
- 4.1.4 Tus nqi ntws: 0.5 mL / feeb.
- 4.1.5 Lub sijhawm nrhiav pom: 30 feeb.
- 4.1.6 Qhov ntim ntawm cov qauv txhaj tshuaj: 20μL.
- 4.1.7 Kub ntawm kem: kub hauv chav.
- 4.1.8 Yuav kom ua rau lub kaw lus chromatographic ua tau raws li qhov yuav tsum tau ua kom pom tseeb, nws tau teev tseg tias nyob rau hauv cov xwm txheej chromatographic saum toj no, qhov ua tau zoo ntawm cov kem chromatographic gel, piv txwv li, tus lej theoretical ntawm cov phaj (N), tsis tsawg dua 10000 xam raws li cov ncov ntawm tus qauv tripeptide (Glycine-Glycine-Glycine).
- 4.2 Kev tsim cov qauv ntsuas molecular mass
- Cov qauv sib txawv ntawm cov molecular mass peptide uas muaj qhov sib txawv ntawm 1 mg / mL tau npaj los ntawm kev sib phim theem txawb, sib xyaw ua ke hauv qee qhov sib piv, thiab tom qab ntawd lim los ntawm cov organic theem membrane nrog qhov loj ntawm 0.2 μm ~ 0.5 μm thiab txhaj rau hauv cov qauv, thiab tom qab ntawd cov chromatograms ntawm cov qauv tau txais. Cov kab sib npaug ntawm cov molecular mass calibration thiab lawv cov kab zauv tau txais los ntawm kev plotting logarithm ntawm cov molecular mass tiv thaiv lub sijhawm khaws cia lossis los ntawm linear regression.
4.3 Kev kho cov qauv
Muab 10mg ntawm cov qauv ntsuas rau hauv lub raj mis volumetric 10mL, ntxiv me ntsis mobile phase, thiab co ultrasonic rau 10 feeb, kom cov qauv yaj tag thiab sib xyaw, diluted nrog mobile phase rau ntawm qhov ntsuas, thiab tom qab ntawd lim los ntawm daim nyias nyias organic phase nrog qhov loj ntawm 0.2μm ~ 0.5μm, thiab cov lim dej tau soj ntsuam raws li cov xwm txheej chromatographic hauv A.4.1.
- 5. Kev suav ntawm kev faib tawm ntawm cov pawg molecular sib piv
- Tom qab tshuaj xyuas cov kua qauv uas tau npaj tseg hauv 4.3 nyob rau hauv cov xwm txheej chromatographic ntawm 4.1, qhov hnyav molecular ntawm cov qauv thiab nws qhov kev faib tawm tuaj yeem tau txais los ntawm kev hloov cov ntaub ntawv chromatographic ntawm cov qauv rau hauv qhov nkhaus calibration 4.2 nrog GPC cov ntaub ntawv ua software. Kev faib tawm ntawm cov hnyav molecular ntawm cov peptides sib txawv tuaj yeem suav los ntawm txoj kev ua kom thaj chaw siab tshaj plaws, raws li cov mis: X = A / A tag nrho × 100
- Hauv cov mis: X - Qhov feem pua ntawm cov molecular mass peptide hauv tag nrho cov peptide hauv cov qauv,%;
- A - Thaj chaw siab tshaj plaws ntawm cov peptide molecular mass;
- Tag Nrho A - qhov sib sau ua ke ntawm cov cheeb tsam siab tshaj plaws ntawm txhua tus peptide molecular mass, suav rau ib qho chaw decimal.
- 6 Rov ua dua
- Qhov sib txawv kiag li ntawm ob qhov kev txiav txim siab ywj pheej uas tau txais raws li cov xwm txheej ntawm kev rov ua dua yuav tsum tsis pub tshaj 15% ntawm qhov nruab nrab ntawm kev suav lej ntawm ob qhov kev txiav txim siab.
- Cov Lus Qhia Ntxiv B: Cov Txheej Txheem rau Kev Txheeb Xyuas Cov Amino Acids Dawb
- Kev txais yuav tus qauv: Q/320205 KAVN05-2016
- 1.2 Cov tshuaj reagents thiab cov ntaub ntawv
- Glacial acetic acid: analytically pure
- Perchloric acid: 0.0500 mol/L
- Qhov Qhia: 0.1% crystal violet indicator (glacial acetic acid)
- 2. Kev txiav txim siab ntawm cov amino acids dawb
Cov qauv tau qhuav ntawm 80 ° C rau 1 teev.
Muab cov qauv tso rau hauv lub thawv qhuav kom txias mus rau qhov kub hauv chav lossis txias mus rau qhov kub uas siv tau.Hnyav kwv yees li 0.1 g ntawm cov qauv (qhov tseeb txog 0.001 g) rau hauv lub raj mis 250 mL qhuav conical.Ua sai sai mus rau kauj ruam tom ntej kom tsis txhob muaj cov qauv nqus cov dej noo ib puag ncigNtxiv 25 mL ntawm glacial acetic acid thiab sib tov kom zoo tsis pub tshaj 5 feeb.Ntxiv 2 tee ntawm cov xim crystal violet indicatorTitrate nrog 0.0500 mol / L (± 0.001) tus qauv titration kua ntawm perchloric acid kom txog thaum cov kua hloov ntawm xim av mus rau qhov kawg.
Sau qhov ntim ntawm cov kua txheem uas tau noj.
- Ua qhov kev xeem dawb paug tib lub sijhawm.
- 3. Kev suav thiab cov txiaj ntsig
- Cov amino acid dawb uas muaj nyob hauv X hauv cov reagent yog qhia ua feem pua (%) thiab suav raws li cov mis: X = C × (V1-V0) × 0.1445/M × 100%, hauv cov mis tne:
- C - Kev sib xyaw ntawm cov kua qaub perchloric acid hauv moles ib liter (mol/L)
- V1 - Ntim siv rau titration ntawm cov qauv nrog tus qauv perchloric acid kua, hauv milliliters (mL).
- Vo - Ntim siv rau titration dawb nrog tus qauv perchloric acid kua, hauv milliliters (mL);
M - Qhov hnyav ntawm cov qauv, hauv grams (g).
| 0.1445: Qhov hnyav nruab nrab ntawm cov amino acids sib npaug rau 1.00 mL ntawm cov kua qaub perchloric acid txheem [c (HClO4) = 1.000 mol / L]. | 4.2.3 Cerium sulfate tus qauv titration kua: concentration c [Ce (SO4) 2] = 0.1 mol/L, npaj raws li GB/T601. | |
| Kev txais yuav cov qauv: Q/70920556 71-2024 | 1. Txoj cai txiav txim siab (Fe ua piv txwv) | Cov amino acid hlau complexes muaj solubility tsawg heev hauv anhydrous ethanol thiab cov hlau ions dawb yog soluble hauv anhydrous ethanol, qhov sib txawv ntawm solubility ntawm ob qho hauv anhydrous ethanol tau siv los txiav txim siab qhov chelation rate ntawm amino acid hlau complexes. |
| Hauv cov mis: V1 - ntim ntawm cerium sulfate tus qauv kua siv rau titration ntawm kev sim kua, mL; | Anhydrous ethanol; tus so yog tib yam li kab lus 4.5.2 hauv GB / T 27983-2011. | 3. Cov kauj ruam ntawm kev tshuaj xyuas |
| Ua ob qhov kev sim ua ke. Hnyav 0.1g ntawm cov qauv qhuav ntawm 103 ± 2 ℃ rau 1 teev, raug rau 0.0001g, ntxiv 100mL ntawm anhydrous ethanol kom yaj, lim, lim cov seem ntxuav nrog 100mL ntawm anhydrous ethanol tsawg kawg peb zaug, tom qab ntawd hloov cov seem rau hauv lub raj mis conical 250mL, ntxiv 10mL ntawm sulfuric acid kua raws li kab lus 4.5.3 hauv GB / T27983-2011, thiab tom qab ntawd ua cov kauj ruam hauv qab no raws li kab lus 4.5.3 "Sov kom yaj thiab tom qab ntawd cia txias" hauv GB / T27983-2011. Ua qhov kev sim dawb paug tib lub sijhawm. | 4. Kev txiav txim siab ntawm tag nrho cov ntsiab lus hlau | 4.1 Lub hauv paus ntsiab lus ntawm kev txiav txim siab zoo ib yam li kab lus 4.4.1 hauv GB / T 21996-2008. |
4.2. Cov Tshuaj Reagents & Cov Tshuaj
| 4.2.1 Cov kua qaub sib xyaw: Ntxiv 150mL ntawm cov kua qaub sulfuric thiab 150mL ntawm cov kua qaub phosphoric rau hauv 700mL dej thiab sib tov kom zoo. | 4.2.2 Sodium diphenylamine sulfonate qhia txog kev daws teeb meem: 5g / L, npaj raws li GB / T603. | 4.2.3 Cerium sulfate tus qauv titration kua: concentration c [Ce (SO4) 2] = 0.1 mol/L, npaj raws li GB/T601. | |
| 4.3 Cov Kauj Ruam ntawm Kev Tshuaj Xyuas | Ua ob qho kev sim ua ke. Hnyav 0.1g ntawm cov qauv, raug rau 020001g, muab tso rau hauv lub raj mis conical 250mL, ntxiv 10mL ntawm cov kua qaub sib xyaw, tom qab yaj, ntxiv 30ml dej thiab 4 tee ntawm sodium dianiline sulfonate qhia cov kua, thiab tom qab ntawd ua cov kauj ruam hauv qab no raws li kab lus 4.4.2 hauv GB / T21996-2008. Ua qhov kev sim dawb paug tib lub sijhawm. | 4.4 Kev sawv cev ntawm cov txiaj ntsig | Tag nrho cov hlau ntsiab lus X1 ntawm cov amino acid hlau complexes nyob rau hauv cov nqe lus ntawm pawg loj ntawm hlau, tus nqi qhia nyob rau hauv%, tau suav raws li cov mis (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - cerium sulfate tus qauv kua siv rau titration ntawm cov kua dawb paug, mL; | V0 - cerium sulfate tus qauv kua siv rau titration ntawm cov kua dawb paug, mL; | C - Qhov tseeb concentration ntawm cerium sulfate txheem kua, mol/L5. Kev suav cov ntsiab lus hlau hauv chelatesCov ntsiab lus hlau X2 hauv chelate hais txog qhov feem pua ntawm cov hlau, tus nqi qhia hauv%, tau suav raws li cov mis: x2 = ((V1-V2) × C × 0.05585) / m1 × 100 |
| Hauv cov mis: V1 - ntim ntawm cerium sulfate tus qauv kua siv rau titration ntawm kev sim kua, mL; | V2 - cerium sulfate tus qauv kua siv rau titration ntawm cov kua dawb paug, mL;nom1-Qhov hnyav ntawm cov qauv, g. Siv qhov nruab nrab ntawm cov txiaj ntsig kev txiav txim siab sib luag ua cov txiaj ntsig kev txiav txim siab, thiab qhov sib txawv kiag li ntawm cov txiaj ntsig kev txiav txim siab sib luag tsis pub ntau tshaj 0.3%. | 0.05585 - qhov hnyav ntawm cov hlau ferrous qhia hauv grams sib npaug rau 1.00 mL ntawm cerium sulfate txheem kua C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1-Qhov hnyav ntawm cov qauv, g. Siv qhov nruab nrab ntawm cov txiaj ntsig kev txiav txim siab sib luag ua cov txiaj ntsig kev txiav txim siab, thiab qhov sib txawv kiag li ntawm cov txiaj ntsig kev txiav txim siab sib luag tsis pub ntau tshaj 0.3%. | 6. Kev suav tus nqi chelationTus nqi chelation X3, tus nqi qhia hauv %, X3 = X2/X1 × 100Cov Lus Qhia Ntxiv C: Cov Txheej Txheem rau Kev Txheeb Xyuas Zinpro's chelation rate |
Kev txais yuav tus qauv: Q/320205 KAVNO7-2016
1. Cov tshuaj reagents thiab cov ntaub ntawv
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Qhov Ntsuas: 0.1% crystal violet indicator (glacial acetic acid)
2. Kev txiav txim siab ntawm cov amino acids dawb
2.1 Cov qauv tau qhuav ntawm 80 ° C rau 1 teev.
2.2 Muab cov qauv tso rau hauv lub thawv qhuav kom txias mus rau qhov kub hauv chav lossis txias mus rau qhov kub uas siv tau.
2.3 Hnyav kwv yees li 0.1 g ntawm cov qauv (qhov tseeb txog 0.001 g) rau hauv lub raj mis qhuav 250 mL conical
2.4 Ua sai sai mus rau kauj ruam tom ntej kom tsis txhob muaj cov qauv nqus cov dej noo ib puag ncig.
2.5 Ntxiv 25mL ntawm glacial acetic acid thiab sib tov kom zoo tsis pub dhau 5 feeb.
2.6 Ntxiv 2 tee ntawm cov xim qhia xim liab dawb.
2.7 Titrate nrog 0.0500mol/L (±0.001) tus qauv titration kua ntawm perchloric acid kom txog thaum cov kua hloov ntawm xim av mus rau ntsuab rau 15 vib nas this yam tsis hloov xim ua qhov kawg.
2.8 Sau qhov ntim ntawm cov kua dej ib txwm siv.
2.9 Ua qhov kev xeem dawb paug tib lub sijhawm.
- 3. Kev suav thiab cov txiaj ntsig
- Catalan
- Physicochemical parameters
V1 - Ntim siv rau titration ntawm cov qauv nrog tus qauv perchloric acid kua, hauv milliliters (mL).
Vo - Ntim siv rau titration dawb nrog tus qauv perchloric acid kua, hauv milliliters (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Chaw Nyob: No.147 Qingpu Road, Shouan Town, Pujiang County, Chengdu City, Sichuan Province, Suav Teb
Xov tooj: 86-18880477902
Cov khoom
Cov zaub mov tsis muaj organic
- Cov zaub mov organic
- Swahili
- Kev pabcuam tshwj xeeb
- Cov kev sib txuas ceev
Cov Ntaub Ntawv Txog Lub Tuam Txhab
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Nyem rau kev nug | © Copyright - 2010-2025: Tag nrho cov cai raug tseg. | Daim Ntawv Qhia Chaw NRHIAV SAUM TOJ KAWG NKAUS Xov tooj |
| Xov tooj | 86-18880477902 | Cov neeg Javanese | Email |
| 8618880477902 | Suav teb | Fabkis | |
| Bird | Suav teb | Fabkis | German Mev |
| Aquatic animals | Nyiv | Kauslim | Lus Arabic Greek |
| Lus Turkish | Italian | ||
| Ruminant animal g/head day | January 0.75 | Indonesian Neeg Asmeskas Swedish |
Polish
- Basque
- Catalan
- Physicochemical parameters
Hindi
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Bulgarian
- Cebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Croatian
Dutch
| Application object | Urdu Nyab Laj | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Neeg Haiti | Hausa | Kinyarwanda Hmoob Hungarian |
| Piglets and fattening pigs | Igbo | Cov neeg Javanese | Kannada Khmer Kurdish |
| Kyrgyz | Latin | ||
| Bird | 300～400 | 45～60 | Neeg Macedonian Malay Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Norwegian
- Pashto
- Appearance: brownish-yellow granules
- Physicochemical parameters
Serbian
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Swahili
Tajik
Tamil
Telugu
Thaib teb
| Application object | Urdu Nyab Laj | Content in full-value feed (mg/kg) | Efficacy |
| Yiddish | Yoruba | Zulu | Kinyarwanda Oriya Turkmen |
| Uyghur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
